Roche lightcycler 480 operators manual department of cell. Roche lightcycler 480 ii real time pcr system for 96 well. I prepared the twostep cycling experiment with the following conditions. First presented in 2005, the lightcycler 480 realtime pcr system extends.
Refer to user manual part number 4387783 rev c for additional information. The lightcycler 480 system features a versatile, platebased, realtime pcr device that supports mono or multicolor applications. With realtime pcr and especially the roche applied science lightcycler systems. Automatically analyzes and interprets realtime data during either amplification or melting curve analysis.
Lightcycler 480 realtime pcr system gene quantification. Lightcycler 480 instrument operators manual is correct at the time of printing. Enter username and password when the login dialog box appears and click the button to proceed with the login. Has anybody used the light cycler 480 system by roche. The lightcycler 480 software enables users to quickly customize the system to meet different application requirements, from basic manual analysis to. Automate labourintensive interactionsallows technicians to focus on more sophisticated activities. Lightcycler 480 multiwell plate 96, white 5 x 10 plates includes sealing foils catalog no. This lightcyler 480 instrument quick guide was created by the roche. The benchtop instrument is easily customizable to meet changing user. It should help users identify critical steps in their workflows and provide. Turn on the computer linked to the instrument and log on to window xp. However, roche diagnostics gmbh reserves the right to make any changes. Discover the new lightcycler 96 realtime pcr system from roche. The cobas 4800 system offers stateoftheart test menu, fully automated sample preparation, realtime pcr amplificationdetection and easytouse software for multiple sample types the detection of chlamydia.
Lightcycler realtime pcr systems application manual. Start the lightcycler 480 software by doubleclicking the icon. I have a problem with the analysis in lightcycler 480 software. It is a high throughput machine and very user friendly and the roche customer support is highly satisfactory. Enter the username and assigned password to log in to the lightcycler 480 software. Refer to the following chapters for general information and for instructions for tasks any user can perform. Minimise opportunity for sample contaminationincreases sample reproducibility and process reliability. Roche lightcycler 480 ii real time pcr system for 96 well plates 8856 2. However, roche diagnostics gmbh reserves the right to make any changes necessary without notice as part of ongoing product development. Preamblebefore settingup operation of the lightcycler 480 instrument, it is important to readthis operator. The system features the lightcycler 480 instrument, a versatile, platebased realtime pcr device that supports mono or multicolor applications. Lightcycler 480 instrument ii roche applied science. For in vitro diagnostic applications in combination with roche diagnostic reagent kits use manual. The benchtop instrument is easily customizable to meet changing user requirements, and can be integrated into everyday use as an automated highthroughput solution.